The original Full Blooded Italians began in Extreme Championship Wrestling (ECW). In April 1996, at Massacre on Queens Boulevard, J.T. Smith - whose gimmick was that, after sustaining a concussion, he believed himself to be Italian (despite being African American) - announced that he had discovered that Damien Stone was his Sicilian cousin, dubbing him "Little Guido". The partnership between Smith and Little Guido expanded to include multiple other wrestlers, including "Big" Val Puccio, Tracy Smothers and Tommy Rich. The running gag of the group was that most of the wrestlers were obviously not of Italian descent, but they often claimed to be and acted in stereotypical Italian American ways, including none-too-subtle Mafia references and constant hand gestures.
The group imploded when Smothers and Rich departed the company, leaving Sal E. Graziano to manage Little Guido on his own. The group became less of a comedy act and more of a serious tandem until Tony Mamaluke was added to the duo in early 2000. Together, managed by Graziano, the group feuded with Mikey Whipwreck and Yoshihiro Tajiri, a team led by The Sinister Minister, over the ECW World Tag Team Championship. The group vied for number one contendership of the title in the company's dying months.
In 2003, Little Guido had been competing in WWE on the SmackDown brand as Nunzio. After a loss to Rikishi, Nunzio threatened to bring in his "family" to gain revenge. The next week, he directed Chuck Palumbo and Johnny "The Bull" Stamboli to attack Rikishi. This latest incarnation was mainly referred to simply as "The FBI". It was heavily implied to be a mafia organization led by Nunzio. This was further reinforced by video vignettes stylized by stakeout footage showing the trio involved with organized crime in New York City backstreets. Despite the publicity, they had very little success at first. Their fortunes changed after using a gimmick of running an illegal betting ring in the backstage area. This led to a series of feuds with notable wrestlers and tag teams, including Los Guerreros, Chris Benoit, Rhyno, and the APA. The FBI was also used by Mr. McMahon and Jamie Noble (Nunzio's storyline cousin) to attack their rivals. When the group turned into fan favorites, they grew in popularity but just as they were getting over, Palumbo was sent to the Raw brand. This left Nunzio and Stamboli in the group. In November 2004, Palumbo and Stamboli were released, but Nunzio retained his current character.
Vito appeared on the August 6, 2005 episode of Velocity by aiding Nunzio with a blackjack to help him win the Cruiserweight Championship from Paul London. The two mainly competed in singles competition. Unlike previous members of the team, Vito often avoided helping Nunzio to keep himself out of peril. The team initially ended when the two had an argument in the ring. A few weeks later, Nunzio took offense to people thinking their alliance was over, and greater offense to suggestions that Vito was a cross-dresser, but once Nunzio saw the allegations to be true, he ended his ties to Vito.
The F.B.I. from the original Extreme Championship Wrestling reunited briefly at the first-ever One Night Stand pay-per-view event in 2005. Members Tracy Smothers, Tony Mamaluke, Big Guido and J.T. Smith accompanied Little Guido for his match, and then reappeared in the final segment to close the show.
The final WWE incarnation of The FBI debuted at the 2006 One Night Stand event. Little Guido had moved over to the ECW brand to reunite his tag team with Mamaluke. They were now managed by newest member Trinity. Big Guido was also in their corner for the pay-per-view and ECW television debut, but left the company that same week. The tag team mainly served as enhancement talent. Trinity eventually began making appearances without the tag team, and Tony Mamaluke was released from his WWE contract on January 18, 2007, thus disbanding The FBI for good.